Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry
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Wax-2005-Prospective grading.pdf
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Author(s)
Wax, Adam
Pyhtila, John W.
Graf, Robert N.
Nines, Ronald
Boone, Charles W.
Feld, Michael S.
Steele, Vernon E.
Stoner, Gary D.
Dasari, Ramachandra Rao
Date Issued
October 2005
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Wax, Adam, John W. Pyhtila, Robert N. Graf, Ronald Nines, Charles W. Boone, Ramachandra R. Dasari, Michael S. Feld, Vernon E. Steele, and Gary D. Stoner. “Prospective Grading of Neoplastic Change in Rat Esophagus Epithelium Using Angle-Resolved Low-Coherence Interferometry.” Journal of Biomedical Optics 10, no. 5 (2005): 051604. © 2005 Society of Photo-Optical Instrumentation Engineers
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Final published version
Abstract
Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria established in a previous a/LCI study to prospectively grade neoplastic progression. We exploit the depth resolution of a/LCI to exclusively examine the basal layer of the epithelium, approximately 50 to 100 μm beneath the tissue surface, without the need for exogenous contrast agents, tissue sectioning, or fixation. The results of two studies are presented that compare the performance of two a/LCI modalities. Overall, the combined studies show 91% sensitivity and 97% specificity for detecting dysplasia, using histopathology as the standard. In addition, the studies enable the effects of dietary chemopreventive agents, difluoromethylornithine (DFMO) and curcumin, to be assessed by observing modulation in the incidence of neoplastic change. We demonstrate that a/LCI is highly effective for monitoring neoplastic change and can be applied to assessing the efficacy of chemopreventive agents in the rat esophagus.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
http://dx.doi.org/10.1117/1.2102767
